Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 3
Release date: Mar 23 2018
Last update date: Jul 24 2018
Access: Public
Dataset link 10X genomics analysis of adult and larval habenula from the gng8-GFP transgenic line

Experimental Protocol


For larval dataset, multiple gng8-GFP labeled zebrafish brains were dissociated and habenular cells FAC sorted into PBS+1%BSA and immediately loaded into 10X chromium for cell capture. For adult dataset, 5-6 habenulae were cleanly dissected out, dissociated, filtered in PBS+BSA and immediately captured in droplets with 10x platform. Both libraries were then constructed using standard instrutions of the 10x plaform.

Repositories


GEO

GSE109158

ENA

SRP131047

BioProject

PRJNA429936

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