|Number of samples:||3|
|Release date:||Mar 23 2018|
|Last update date:||Jul 24 2018|
|Dataset link||10X genomics analysis of adult and larval habenula from the gng8-GFP transgenic line|
For larval dataset, multiple gng8-GFP labeled zebrafish brains were dissociated and habenular cells FAC sorted into PBS+1%BSA and immediately loaded into 10X chromium for cell capture. For adult dataset, 5-6 habenulae were cleanly dissected out, dissociated, filtered in PBS+BSA and immediately captured in droplets with 10x platform. Both libraries were then constructed using standard instrutions of the 10x plaform.