Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Apr 1 2007
Access: Public
Diseases: Breast Neoplasms, Neoplasms, Hereditary Angioedema Type III
Chemicals: Doxycycline, Estrogens
Genes: LMO4, rpoTp, LDB1, BMP7, HDAC2
Taxon: Homo sapiens, Mus musculus
Dataset link Expression profiling of MCF-7 cells with inducible LMO4 and DN-Clim expression

Experimental Protocol


We used the Tet-off system to establish several distinct MCF-7 cell clones, referred to as MCF7-LMO4-TetOff and MCF7-DN-Clim-TetOff cells, in which the expression of Myc-tagged LMO4 or DN-Clim was repressed by the presence of doxycycline in the medium. Total RNA was isolated from three distinct MCF7-LMO4-TetOff cell lines and three distinct MCF7-DN-Clim-TetOff cell lines in presence and absence of doxycycline after 6 days. Treatment with doxycycline To decrease variability, we pooled RNAs from three experiments for each of the three cell lines.

Repositories


GEO

GSE7382

ArrayExpress

E-GEOD-7382

BioProject

PRJNA100389

Download


Contact


Kevin Lin
Kevin K. Lin