Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Jan 24 2009
Last update date: Aug 28 2018
Access: Public
Diseases: Larva Migrans
Chemicals: Fatty Acids, Triglycerides
Dataset link Expression profiling of HNF4-dependent transcripts in Drosophila larvae

Experimental Protocol


Staged late second instar control larvae that were transheterozygous for precise excisions of the EP2449 and KG08976 P-elements, and dHNF4D33/dHNF4D17 mutant larvae, were either fed or starved for 24 hrs in a 55mm petri dish containing Whatman paper soaked with PBS. RNA was extracted from the apparently healthy animals that molted to the third instar using Trizol (Gibco) and purified on RNAeasy columns (Qiagen). All samples were prepared in triplicate to facilitate subsequent statistical analysis. Probe labeling, hybridization to Affymetrix GeneChip® Drosophila Genome 2.0 Arrays.

Repositories


GEO

GSE14531

ArrayExpress

E-GEOD-14531

BioProject

PRJNA111609

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Contact


Carl Thummel