|Application:||Gene expression microarray analysis|
|Number of samples:||12|
|Release date:||Jan 24 2009|
|Last update date:||Aug 28 2018|
|Chemicals:||Fatty Acids, Triglycerides|
|Dataset link||Expression profiling of HNF4-dependent transcripts in Drosophila larvae|
Staged late second instar control larvae that were transheterozygous for precise excisions of the EP2449 and KG08976 P-elements, and dHNF4D33/dHNF4D17 mutant larvae, were either fed or starved for 24 hrs in a 55mm petri dish containing Whatman paper soaked with PBS. RNA was extracted from the apparently healthy animals that molted to the third instar using Trizol (Gibco) and purified on RNAeasy columns (Qiagen). All samples were prepared in triplicate to facilitate subsequent statistical analysis. Probe labeling, hybridization to Affymetrix GeneChip® Drosophila Genome 2.0 Arrays.