Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 42
Release date: May 8 2015
Last update date: Jan 8 2018
Access: Public
Chemicals: Nitrogen
Computational protocol: KEGG, GenePix Pro, GeneSpring GX, Primer3, QuickGO, BLASTN, OligoCalc, GEO, UniProtKB, COGs, CDD
Dataset link Microarray transcriptional profiling of Arctic Mesorhizobium strain N33 at low temperature provides insights into cold adaption strategies

Experimental Protocol


The analysis used Arctic strain N33 (growth at room temperature) RNA as control (reference) samples for comparison to the experimental samples collected under constant growth at 4°C and 10°C, and five different cold treatment conditions at 2, 4, 8, 60, and 240 min (three biological replicates for each treatment). Dual-dye hybridizations were performed including three biological replicates and two technical replicates (dye flip, Cy5/Cy3) for each treatment for a total of 42 array hybridizations and 84 labelling reactions

Repositories


GEO

GSE60710

BioProject

PRJNA259352

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Contact


Linda Harris

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