Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 70
Release date: Jul 25 2018
Last update date: Nov 29 2018
Access: Public
Diseases: Alzheimer Disease, Amyloidosis, Neurodegenerative Diseases, Tauopathies
Dataset link Microglial translational profiling reveals a convergent APOE pathway from aging, amyloid, and tau

Experimental Protocol


Microglial transcriptomes from the forebrain were generated using RNAseq using an Illumina HiSeq 4000 were done for N=3-5 animals per condition in the context of aging, amyloidosis, tauopathy, and systemic inflammation (i.e. LPS or Poly(I:C) stimulation) in RiboTag mice. In addition, a cellular isolation procedure using enzymatic digestion and cell sorting was also used to compare this protocol versus RiboTag isolation during basal conditions and LPS induced inflammation.

Repositories


GEO

GSE117646

ENA

SRP155199

BioProject

PRJNA482826

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