Dataset features


Application: Gene expression microarray analysis
Number of samples: 10
Release date: Apr 8 2014
Last update date: Apr 8 2014
Access: Public
Diseases: Alzheimer Disease, Bacterial Infections, Endometritis, Mastitis
Chemicals: Fatty Acids, Fatty Acids, Nonesterified, Oxygen
Dataset link Negative energy balance and splenic gene expression patterns in high yielding dairy cows during the early postpartum period [spleen]

Experimental Protocol

Multiparous Holstein-Friesian cows (n=24) were blocked 2 weeks prior to expected calving date according to parity, body condition score, and previous lactation yield (average lactation 6477±354kg) and randomly allocated to mild (MNEB; n=12) or severe (SNEB; n=12) NEB groups. MNEB cows were fed ad lib grass silage and 8 kg day-1 concentrates and milked once daily; SNEB cows were fed 25 kg day-1 silage and 4kg day-1 concentrate and milked three times daily. Measurements of body condition score and EB were used to select cows which showed extremes in EB from each group (MNEB, n=5; SNEB, n=5). Cows were slaughtered on days 6-7 of the first follicular wave after calving (mean number of days post-partum: MNEB mean 13.6 ± 0.75, range 11–15; SNEB mean 14.3 ± 0.56, range 13–16), based on daily transrectal ultrasonography.At slaughter the entire spleen was removed and weighed, and samples weighing 1 g were dissected, rinsed in RNase-free phosphate buffer, snap frozen in liquid nitrogen, stored for 4 h in dry ice, and subsequently stored at 80°C.










Dermot Morris
Dermot G Morris

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