Computational protocol: Molecular Cloning, Expression Profile and 5′ Regulatory Region Analysis of Two Chemosensory Protein Genes from the Diamondback Moth, Plutella xylostella

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Protocol publication

[…] Amino acid sequences of CSPs (n = 27) were retrieved from an NCBI protein search using the keywords “chemosensory protein” and “lepidopteran”. Molecular mass and isoelectric point was predicted using the software, ExPASy ( Multiple sequence alignment was carried out with the online service at (). Promoter prediction and characterization were carried out using the Neural Network Promoter Prediction (NNPP) server ( (). Sequence analysis seeking transcriptional regulation response elements was carried out with TFSEARCH ( (). The signal peptide was predicted using SignalP 3.0 () at The phylogenetic tree was constructed using MEGA 3.0 software () using the neighbour joining method, and it was reconstructed with 1000-replicate bootstrap analysis. […]

Pipeline specifications

Software tools MultAlin, NNPP, TFSearch, SignalP, MEGA
Databases NCBI Protein ExPASy
Applications Genome annotation, Phylogenetics
Organisms Plutella xylostella