Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 4
Release date: May 7 2018
Last update date: Aug 6 2018
Access: Public
Diseases: Precursor B-Cell Lymphoblastic Leukemia-Lymphoma
Dataset link The oligodendrocyte "protrusiosome": Identification of compartmentalized enrichment of transcripts in newly-formed protrusions of oligodendrocyte precursor cells (OPC) by RNAseq

Experimental Protocol


Rat OPCs were plated on a modified Boyden chamber system for 24h consisting of a transwell with 1μm pore size with chemo- and haptotactic gradients to physically separate OPC soma and protrusion fractions for RNA sequencing. RNAseq was done on 2 samples for OPC soma and 2 samples of OPC protrusions. Due to very low RNA yields in OPC protrusions samples (typically 9ng per well), each sample consisted in a pool of several experiments.

Repositories


GEO

GSE106862

ENA

SRP124934

BioProject

PRJNA418248

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Contact


Helena Domingues