|Number of samples:||16|
|Release date:||Apr 5 2018|
|Last update date:||Nov 21 2018|
|Dataset link||RNA-seq analysis of osteogenic differentiating mouse wild type (WT) and Zfp384 (Nmp4) knockout mesenchymal stem/progenitor cells (MSPCs)|
To compare transcriptome profiles of non-differentiating and osteogenic-differentiating WT and Nmp4-/- MSPCs, cells were seeded into 12-well plates at either 10,000 cells/well (25 cells/mm2) or 25,000 cells/well (62 cells/mm2). The cells seeded at the lower density were maintained in Mesencult MediaTM + Mesencult Stimulatory SupplementTM (non-differentiating medium) for 3 days post-seeding and then harvested for total RNA. Cells plated at the higher density were maintained in Î±-MEM complete medium throughout the experiment. At 48 hours post-seeding the medium was refreshed with the ascorbic acid, dexamethasone, and BGP supplement. These cells were harvested at 7 days post-seeding as early osteogenic cells. For each condition there were four technical replicates and 1 biological replicate.