Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 16
Release date: Apr 5 2018
Last update date: Nov 21 2018
Access: Public
Diseases: Osteoporosis
Dataset link RNA-seq analysis of osteogenic differentiating mouse wild type (WT) and Zfp384 (Nmp4) knockout mesenchymal stem/progenitor cells (MSPCs)

Experimental Protocol


To compare transcriptome profiles of non-differentiating and osteogenic-differentiating WT and Nmp4-/- MSPCs, cells were seeded into 12-well plates at either 10,000 cells/well (25 cells/mm2) or 25,000 cells/well (62 cells/mm2). The cells seeded at the lower density were maintained in Mesencult MediaTM + Mesencult Stimulatory SupplementTM (non-differentiating medium) for 3 days post-seeding and then harvested for total RNA. Cells plated at the higher density were maintained in α-MEM complete medium throughout the experiment. At 48 hours post-seeding the medium was refreshed with the ascorbic acid, dexamethasone, and BGP supplement. These cells were harvested at 7 days post-seeding as early osteogenic cells. For each condition there were four technical replicates and 1 biological replicate.

Repositories


GEO

GSE112694

ENA

SRP137142

BioProject

PRJNA448750

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Contact


Joseph Bidwell