Dataset features

Specifications


Application: BS-seq analysis
Number of samples: 6
Release date: Oct 1 2016
Last update date: Nov 28 2018
Access: Public
Taxon: Homo sapiens
Dataset link Differential DNA Methylation Regions in Adult Human Sperm following Adolescent Chemotherapy: Potential for Epigenetic Inheritance

Experimental Protocol


DNA from the semen samples was isolated and equal amounts of DNA from 3 individuals pooled to generate three different pools of control (no previous chemotherapy) and three different pools of adolescent chemotherapy exposed cancer survivors labeled human sperm pools (HS#) #1 through #6. The pooled DNA was immunoprecipitated with 5 methyl cytosine monoclonal antibody for a methylated DNA immunoprecipitation (MeDIP). The MeDIP DNA was used to generate libraries and bar coded (index primers) separately for analysis by next generation sequencing (MeDIP-Seq).

Repositories


GEO

GSE85790

ENA

SRP082309

BioProject

PRJNA339396

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Contact


Michael Skinner