Computational protocol: Comparison of Algorithms for the Detection of Enteroviruses in Stool Specimens from Children Diagnosed with Acute Flaccid Paralysis

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Protocol publication

[…] The CLUSTAL W programme in MEGA 5 software [] was used with default settings to align sequences of the enterovirus type(s) whose Nigerian strains were first described in this study alongside those retrieved from GenBank. Subsequently, a neighbor-joining tree was constructed using the same MEGA5 software [] with the Kimura-2 parameter model [] and 1000 bootstrap replicates. The accession numbers of sequences retrieved from GenBank for this analysis are indicated in the sequences name on the phylograms. […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics
Organisms Homo sapiens