Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Aug 2 2011
Last update date: Aug 15 2018
Access: Public
Dataset link Expression data from Arabidopsis thaliana under dark and far-red light

Experimental Protocol


We used the FHY3p:FHY3-GR fhy3-4 transgenic lines expressing FHY3-glucocorticoid receptor (GR) fusion proteins under the control of the FHY3 native promoter, in which, without dexamethasone (DEX), the GR-fusion proteins localize in the cytoplasm, whereas, when treated with DEX, the fusion proteins translocate into the nucleus. The seedlings were grown in D or continuous FR light for 4 days, then treated with DEX or mock (equal volume of ethanol), and grown in the same conditions for a further 2 hours before the samples were harvested. RNA samples were extracted from these samples, and then hybridized with Affymetrix Arabidopsis ATH1 genome arrays. Four independent biological replicates for each treatment were used for the microarray analysis.

Repositories


GEO

GSE30712

ArrayExpress

E-GEOD-30712

BioProject

PRJNA154685

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Contact


Bosheng Li