|Application:||Gene expression microarray analysis|
|Number of samples:||3|
|Release date:||Jan 28 2009|
|Last update date:||Mar 19 2012|
|Dataset link||Protein Engineering of the Quorum-Sensing Regulator SdiA of Escherichia coli|
For the microarray experiments, 10 g glass wool (Corning Glass Works, Corning, N.Y.) were used to form biofilms in 250 mL LB ( 1 mM IPTG + Cm30) in 1 L Erlenmeyer shake flasks which were inoculated with overnight cultures diluted that were 1:100. The cells were shaken at 250 rpm and 30C for 12 hours to form biofilms on the glass wool, and RNA was isolated from the the biofilm cells.