Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 3
Release date: Jan 28 2009
Last update date: Mar 19 2012
Access: Public
Taxon: Escherichia coli
Dataset link Protein Engineering of the Quorum-Sensing Regulator SdiA of Escherichia coli

Experimental Protocol


For the microarray experiments, 10 g glass wool (Corning Glass Works, Corning, N.Y.) were used to form biofilms in 250 mL LB ( 1 mM IPTG + Cm30) in 1 L Erlenmeyer shake flasks which were inoculated with overnight cultures diluted that were 1:100. The cells were shaken at 250 rpm and 30C for 12 hours to form biofilms on the glass wool, and RNA was isolated from the the biofilm cells.

Repositories


GEO

GSE11779

ArrayExpress

E-GEOD-11779

BioProject

PRJNA105969

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Contact


Jintae Lee