Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Apr 7 2010
Last update date: Mar 22 2012
Access: Public
Diseases: Pyruvate Carboxylase Deficiency Disease
Chemicals: Phosphatidylcholines
Dataset link Transcriptomic characterization of a virulence-deficient phosphatidylcholine-negative Agrobacterium tumefaciens mutant

Experimental Protocol


Total RNA was extracted from A. tumefaciens C58 wild-type and PC-deficient mutant cells grown under virulence-induced and non-induced conditions. The 4-plex NimbleGen Gene Expression Array for A. tumefaciens (Design ID 080630) represents 5344 genes and is based on NCBI reference sequences NC_003062, NC_003063, NC_003064 and NC_003065. Each gene is represented by 6 probes (45mer-60mer oligonucleotides), each in two copies on different locations on the array. Three biological replicates for each cultivation condition were analyzed for A. tumefaciens wild-type and PC-deficient mutant strains.

Repositories


GEO

GSE21223

ArrayExpress

E-GEOD-21223

BioProject

PRJNA126483

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Contact


sonja klüsener