Dataset features

Specifications


Application: BS-seq analysis
Number of samples: 6
Release date: Jul 20 2017
Last update date: Nov 21 2018
Access: Public
Chemicals: Ethanol
Dataset link DNA Methylation alterations following binge-like developmental ethanol exposure assessed in 21 day-old mice

Experimental Protocol


Matched male littermates were injected with either ethanol or saline on postnatal day 4 and 7, hippocampal DNA was isolated on postnatal day 21. DNA methylation was assessed for 3 ethanol-treated and 3 control mice via MeDIP-Seq by Arraystar Inc. Methylated DNA was immunoprecipitated, and fragments were sequenced by running 150 cycles on Illumina HiSeq 2000. Reads were aligned to the mouse genome (mm10) using Bowtie. MeDIP enriched regions (peaks) were identified by MASC v2 using a q-value threshold of 10^-4.

Repositories


GEO

GSE101636

ENA

SRP112895

BioProject

PRJNA395044

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Contact


Shiva Singh