Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: May 1 2008
Last update date: Jan 18 2013
Access: Public
Diseases: Macrophage Activation Syndrome
Chemicals: Arsenic, Copper, Oxygen, Zinc
Genes: MT1F, IL6
Dataset link INDUCTION OF OXIDATIVE STRESS AND METAL BINDING GENES IN HUMAN ALVEOLAR MACROPHAGES EXPOSED TO FINE PARTICLES

Experimental Protocol


Total RNA was isolated from alveolar macrophages from 6 subjects were treated in vitro with PM2.5 particles or vehicle control for 4 hrs. All particle-treated samples were co-hybridized with the common reference sample (pool of all 6 vehicle-treated samples) twice with Cy3 and Cy5 dyes flipped (12 hybridizations). Three samples were also co-hybridized with its own individual control (vehicle-treatment for the same subject) with Cy3 and Cy5 dyes flipped (6 additional hybridizations).

Repositories


GEO

GSE10394

ArrayExpress

E-GEOD-10394

BioProject

PRJNA108145

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Contact


Ivana Yang
Ivana V Yang