Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 15
Release date: Sep 1 2008
Last update date: Mar 3 2017
Access: Public
Diseases: Infarction, Posterior Cerebral Artery
Dataset link Molecular Mechanisms of Early Response in Adaptive Cerebral Arteriogenesis

Experimental Protocol


Using an established rat model of nonischemic cerebral hypoperfusion (3-VO) (Busch, Buschmann; 2003), RNA was extracted from isolated ipsilateral PCA. Pooled RNAs from groups of intact (0h), sham and 3-VO animals 24h and 3 days after surgery, were hybridised repeatedly for an extensive genome screen of 15866 genes applying standardized Affymetrix technology. For each Array total RNA from 8 animals was processed, pooled and hybridized to a Rat230A GeneChip per group. These groups were classified as follows: intact control (N=3), 24h3VO (N=3); 24h sham (N=3), 3days3VO (N=3); and 3days sham(N=3). Hybridization, washing, antibody amplification, staining, and scanning of probe arrays were performed according to the Affymetrix Technical Manual. Probe arrays were scanned using the GeneChip System (Hewlett-Packard, Santa Cruz, CA)(Affymetrix) and raw data were processed using GCOS and normalized to a global intensity of 500.

Repositories


GEO

GSE6189

ArrayExpress

E-GEOD-6189

BioProject

PRJNA100577

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Contact


Philipp Hillmeister
Philipp A.M. Hillmeister