Computational protocol: Increased complexity of mushroom body Kenyon cell subtypes in the brain is associated with behavioral evolution in hymenopteran insects

Similar protocols

Protocol publication

[…] Whole brains were embedded in Tissue-Tek OCT compound (Sakura Finetek Japan) without fixation and immediately frozen. In situ hybridization was performed essentially as described previously. For A. reticulata samples with Trp probes, signal amplification using the TSA biotin system (PerkinElmer) was performed between washing and color development. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Images were captured with a light microscope BX-50 (Olympus), merged using GIMP2.8 software (http://www.gimp.org/), and then the brightness and contrast was adjusted using ImageJ 64. […]

Pipeline specifications

Software tools GIMP, ImageJ
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Apis mellifera, Vespa mandarinia