Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 93
Release date: Jan 4 2010
Last update date: Mar 19 2012
Access: Public
Genes: SNCA
Taxon: Saccharomyces cerevisiae, Homo sapiens
Dataset link Rescue of alpha-synuclein toxicity in a yeast model of Parkinson's disease

Experimental Protocol


For the time series, at each time point (0, 2, 4, 6 hours) three yeast samples (2-copy alpha-synuclein; 1-copy alpha-synuclein; empty vector control) were each prepared and hybridized in all three combinations (2-copy vs control; 1-copy vs control; 2-copy vs 1-copy) for that time point. For the drug treatment, three yeast samples were grown each with 2-copy alpha-synuclein or an empty vector control, with or without one of six compounds: compound 1 ((4-(3-iodophenyl)-3,4-dihydrobenzo[h]quinolin-2(1H)-one); compound 2 (4-(3-bromophenyl)-3,4-dihydrobenzo[h]quinolin-2(1H)-one); compound 3 (4-(5-bromo-2-fluorophenyl)-6,7-dimethyl-3,4-dihydro-2(1H)-quinolinone); compound 4 (4-(3-bromo-4-fluorophenyl)-6,7-dimethyl-3,4-dihydro-2(1H)-quinolinone); compound 5 (4-(4-ethyl)-6,7-dimethyl-3,4-dihydro-2(1H)-quinolinone); compound 6 ((4R)-6-bromo-4-(4-ethylphenyl)-3,4-dihydrobenzo[h]quinolin-2(1H)-one). 2-color hybridization compared (a) 2-copy vs 2-copy+compound, (b) vector vs vector+compound, or (c) 2-copy+compound vs vector+compound.

Repositories


GEO

GSE11633

BioProject

PRJNA106153

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