|Number of samples:||8|
|Release date:||Oct 28 2009|
|Last update date:||Mar 21 2012|
|Taxon:||Salmonella enterica subsp. enterica serovar Typhimurium|
|Dataset link||Identification of StpA-binding sites on the Salmonella genome|
To identify the direct StpA targets, we co-immunoprecipitated DNA from a strain expressing a 3xFLAG-tagged StpA-protein using an anti-3xFLAG antibody. As a negative control, we used the same antibody (anti-3xFLAG) to co-immunoprecipitate DNA from an isogenic strain expressing the non-tagged StpA-protein. Three independent biological replicates were generated. For comparative purposes we also performed the co-immunoprecipitation on wild type Salmonella using a monoclonal anti-H-NS antibody. In that case, the negative control was obtained by performing the co-immunoprecipitation protocol on wild type Salmonella without adding antibody.