Dataset features

Specifications


Application: ChIP-on-chip analysis
Number of samples: 8
Release date: Oct 28 2009
Last update date: Mar 21 2012
Access: Public
Genes: stpA
Taxon: Salmonella enterica subsp. enterica serovar Typhimurium
Dataset link Identification of StpA-binding sites on the Salmonella genome

Experimental Protocol


To identify the direct StpA targets, we co-immunoprecipitated DNA from a strain expressing a 3xFLAG-tagged StpA-protein using an anti-3xFLAG antibody. As a negative control, we used the same antibody (anti-3xFLAG) to co-immunoprecipitate DNA from an isogenic strain expressing the non-tagged StpA-protein. Three independent biological replicates were generated. For comparative purposes we also performed the co-immunoprecipitation on wild type Salmonella using a monoclonal anti-H-NS antibody. In that case, the negative control was obtained by performing the co-immunoprecipitation protocol on wild type Salmonella without adding antibody.

Repositories


GEO

GSE18450

ArrayExpress

E-GEOD-18450

BioProject

PRJNA123325

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Contact


Sacha Lucchini