|Application:||DNA methylation array analysis, ChIP-on-chip analysis|
|Number of samples:||21|
|Release date:||Dec 31 2008|
|Last update date:||Dec 6 2012|
|Diseases:||Intestinal Neoplasms, Neoplasms, Inflammatory Bowel Diseases|
|Dataset link||Methylation of Polycomb target genes in intestinal cancer is mediated by inflammation|
In our study we used mice Gpx1/2-ko with B6 and B6.129 genetic background which are characterized by different level of inflammation in ileum and different tumor susceptibility. DNA methylation was analyzed by using MIRA-assisted Microarrays approach. Changing of the DNA methylation in intestinal epithelium during inflammation and tumorgenesis in Gpx1/2-KO mice were compared to healthy control mice with matching age. Aged dependent DNA methylation was study by comparing DNA methylation between 28-days-old healthy control mice with 8-month-old mice. To detect DKO-specific DNA methylation, we compared the changing DNA methylation in liver from Gpx1/2-KO mice with liver from control mice at age 28 days and 8 month. Association of the inflammation dependent DNA methylation with Polycomb targets and loss of H3K27me3 were verified by ChIp-on-ChIp experiments using H3K27me3 antibodies.