Dataset features

Specifications


Application: DNA methylation array analysis, ChIP-on-chip analysis
Number of samples: 21
Release date: Dec 31 2008
Last update date: Dec 6 2012
Access: Public
Diseases: Intestinal Neoplasms, Neoplasms, Inflammatory Bowel Diseases
Chemicals: Glutathione
Dataset link Methylation of Polycomb target genes in intestinal cancer is mediated by inflammation

Experimental Protocol


In our study we used mice Gpx1/2-ko with B6 and B6.129 genetic background which are characterized by different level of inflammation in ileum and different tumor susceptibility. DNA methylation was analyzed by using MIRA-assisted Microarrays approach. Changing of the DNA methylation in intestinal epithelium during inflammation and tumorgenesis in Gpx1/2-KO mice were compared to healthy control mice with matching age. Aged dependent DNA methylation was study by comparing DNA methylation between 28-days-old healthy control mice with 8-month-old mice. To detect DKO-specific DNA methylation, we compared the changing DNA methylation in liver from Gpx1/2-KO mice with liver from control mice at age 28 days and 8 month. Association of the inflammation dependent DNA methylation with Polycomb targets and loss of H3K27me3 were verified by ChIp-on-ChIp experiments using H3K27me3 antibodies.

Repositories


GEO

GSE12315

ArrayExpress

E-GEOD-12315

BioProject

PRJNA113543

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Contact


Maria Hahn