Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Apr 14 2018
Last update date: Apr 16 2018
Access: Public
Diseases: Respiratory Tract Infections
Dataset link The miRNA-targeted transcriptome of porcine alveolar macrophages upon infection with Porcine Reproductive and Respiratory Syndrome Virus (total RNA)

Experimental Protocol


Lung alveolar macrophages were obtained by lung lavage from 4 animals. Cells were infected in vitro with the strain Finistère at multiplicity of infection of 2, or mock infected. Infected cells were harvested at 7 and 10 hours post-infection; control (mock infected) cells were collected at the same times. Total RNAs were extracted from total cell contents and from RISC (RNA-induced Silencing Complex) immunoprecipitates. RNAs were hybridized on microarrays and transcripts analyzed for relative enrichment in RISC (RIP-Chip technology).

Repositories


GEO

GSE98340

BioProject

PRJNA384777

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Contact


Marco Moroldo