Dataset features

Specifications


Application: CLIP-seq analysis
Number of samples: 8
Release date: Apr 21 2016
Last update date: Oct 24 2018
Access: Public
Chemicals: Guanidine
Mutations: p|SUB|R|534|H
Computational protocol: STAR, NGSUtils
Dataset link Profile of RNAs bound by wild-type (WT) or R534H variant of DDX3 with or without Sodium Arsenite treatment [iCLIP-Seq]

Experimental Protocol


Cells were treated with or without sodium arsenite. RNAs crosslinked by UV to FLAG-tagged DDX3 WT or DDX3 R534H were immunoprecipitated sequentially, first with anti-FLAG antibody and second with anti-DDX3 antibody, then sequenced.

Repositories


GEO

GSE70801

ENA

SRP060675

BioProject

PRJNA289566

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Contact


Sekyung Oh

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