|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Oct 30 2018|
|Last update date:||Nov 1 2018|
|Genes:||hop, Stat92E, EGFR, Egfr, LGALS4|
|Dataset link||Drosophila egg chambers: control vs. ectopic JAK/STAT and EGFR signaling|
A reference design was used to control for dye bias in a two-color experiment. Reference (WT) RNA was isolated from the ovaries of OreR flies. Experimental RNA was isolated from the ovaries of 5-8 day old flies of genotype GAL80TS/+ ; GR1-GAL4/UAS-Unpaired, UAS-λtorpedo and control RNA from ovaries of 5-8 day old flies of genotype GAL80TS/+ ; GR1-GAL4/TM6B. Three biological replicates each were performed for the experimental and control conditions. A total of 6 arrays were used. Experimental and control RNA was labeled with Cy5 and universal common reference RNA with Cy3.