Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Oct 30 2018
Last update date: Nov 1 2018
Access: Public
Genes: hop, Stat92E, EGFR, Egfr, LGALS4
Taxon: Drosophila melanogaster
Dataset link Drosophila egg chambers: control vs. ectopic JAK/STAT and EGFR signaling

Experimental Protocol


A reference design was used to control for dye bias in a two-color experiment. Reference (WT) RNA was isolated from the ovaries of OreR flies. Experimental RNA was isolated from the ovaries of 5-8 day old flies of genotype GAL80TS/+ ; GR1-GAL4/UAS-Unpaired, UAS-λtorpedo and control RNA from ovaries of 5-8 day old flies of genotype GAL80TS/+ ; GR1-GAL4/TM6B. Three biological replicates each were performed for the experimental and control conditions. A total of 6 arrays were used. Experimental and control RNA was labeled with Cy5 and universal common reference RNA with Cy3.

Repositories


GEO

GSE118881

BioProject

PRJNA487095

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Contact


Julia Wittes