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[…] g was set to 0.2 min of isothermal heating at 70 °C, followed by 10 °C/min ramp to 270 °C, a 4.0 min isothermal heating of 270 °C, 20 °C/min ramp to 320 °C, and a 2.0 min isothermal heating of 320 °C as previously described. Electron impact ionization (70 eV) at full scan mode (m/z 40–600) was used, with an acquisition rate of 30 spectra/sec. Fatty acid methyl esters (C4–C24 FAMEs; Sigma-Aldrich), alkanes (alkane standard mix [C10–C40]; Sigma-Aldrich), and quality control standards (oxalic acid, malonic acid, malic acid, citric acid, methionine, and 2-butenedioic acid) were run to ensure reproducibility of retention indices and derivatization. Data was processed, aligned, and analyzed using ChromaTOF v. with the statistical compare function (Leco, St. Joseph, MI), Metaboanalyst, and in house software MetaboLyzer as previously described., Cells were seeded at a density of 1,000 cells per well in 3, 96-well plastic tissue culture dishes per cell line on day 0. On day 1, one plate was stained with crystal violet (Sigma,C0775). For staining, plates were rinsed 1 time with 1X PBS to remove excess cellular debris. After, 100 µL of 3.2% PFA was added to each well and incubated at room temperature for 5 minutes followed by three washes in 1X PBS. Then, 200 µL of 0.5% crystal violet in 25% methanol was added to each well and incubated at 4 C for 10 min. The stain was then removed and the plate was rinsed 4–6 × with diH2O to remove excess stain. The plates were left to air-dry overnight. On day 8 […]

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