Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Apr 20 2017
Last update date: Mar 8 2018
Access: Public
Diseases: Hyperglycemia, Hypoglycemia, Hereditary Angioedema Types I and II
Chemicals: Calcium, Glucose, Sulfonylurea Compounds
Dataset link Glucose sensing by skeletal myocytes couples nutrient signaling to systemic homeostasis

Experimental Protocol


C2C12 myoblasts were transduced with retroviruses expressing shRNAs targeting control or mouse Baf60c, differentiated into myotubes, and treated with 1 mM or 10 mM glucose for 12 h. Then cells were harvested for RNA isolation and microarray hybridization using Affymetrix Mouse Gene ST 2.1 Arrays. Two independent biological replicates for each treatment were included in this study.

Repositories


GEO

GSE79925

BioProject

PRJNA317425

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Contact


Zhuoxian Meng