Computational protocol: IQGAP1 in Podosomes/Invadosomes Is Involved in the Progression of Glioblastoma Multiforme Depending on the Tumor Status

Similar protocols

Protocol publication

[…] For red/green colocalization analysis, the open resource digital image analysis software ImageJ has been used (Rasband, W.S., ImageJ, National Institutes of Health, Bethesda, MD, USA,, 1997–2004), implemented with the Manders’ coefficients plug-in developed by Tony Collins (Wright Cell Imaging Facility, Toronto, ON, Canada), which calculates Manders’ coefficients for two 8- or 16-bit images or stacks. Briefly, all confocal images were captured at the same magnification (40×) and with the same levels of contrast and brightness. Using the ImageJ tools, images were then converted in 8-bit grey scale, background subtracted and analyzed for colocalization with “Manders’ coefficients” plug-in. The range value of Manders’ Overlap coefficient (R) is 0–1, with 0 representing low colocalization, and 1 high colocalization. The values obtained were used to create a box and whisker plot to visualize the results. In are reported the mean and standard deviation of the values obtained. […]

Pipeline specifications

Software tools Coloc, ImageJ
Application Microscopic phenotype analysis
Organisms Homo sapiens
Diseases Brain Neoplasms, Glioblastoma, Neoplasms