Computational protocol: Upregulation of a novel eukaryotic translation initiation factor 5A (eIF5A) in dengue 2 virus-infected mosquito cells

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Protocol publication

[…] The similarity of the eIF5A coding sequence derived from Ae. albopictus-derived C6/36 cells was compared, using the basic local alignment search tool [] in the BLAST network service (National Center for Biotechnology Information, Bethesda, MD, USA), against those from selected species (see "Results") in the database. All sequences were aligned using the default parameters of CLUSTAL X [] and edited by Genedoc software []; from this, a phylogenetic analysis using an unrooted tree constructed with the distance-based Neighbor-joining (NJ) method was carried out with MEGA4 []. One thousand bootstrap replications were performed. Other parameters used the default option. […]

Pipeline specifications

Software tools BLASTN, Clustal W, MEGA
Application Phylogenetics
Organisms Dengue virus 2, Homo sapiens, Aedes albopictus
Diseases Dengue, Infection, Virus Diseases, HIV Infections, Severe Dengue
Chemicals Nucleotides