Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 2
Release date: Aug 15 2010
Last update date: Nov 10 2014
Access: Public
Genes: SMU_2080, SMU_2081
Dataset link Identification of a Novel Bacteriocin Regulatory System in Streptococcus mutans

Experimental Protocol


Streptococcus mutans UA140 derivatives, lacA ( genotype: ΔbrsRM /pFW5::PlacA ) and lacA2080 (genotype:ΔbrsRM /pFW5::PlacA-brsR), were cultivated overnight at 37°C in a chemically-defined medium containing 0.5% (wt/vol) glucose as carbon source . The overnight cultures were diluted 1:30 in CDM with 1% (wt/vol) lactose in a total volume of 30 ml. The cells were allowed to grow to an optical density at 600 nm of 0.3 and RNA was extracted. The transcriptional profile of the whole genome was examined with microarray.

Repositories


GEO

GSE22902

ArrayExpress

E-GEOD-22902

BioProject

PRJNA127951

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Contact


Justin Merritt
Justin Merritt