|Application:||Gene expression microarray analysis|
|Number of samples:||10|
|Release date:||Dec 1 2009|
|Last update date:||Oct 29 2018|
|Diseases:||Arthritis, Rheumatoid, Autoimmune Diseases, Common Variable Immunodeficiency|
|Dataset link||Complement receptor 2/CD21– human naive B cells contain mostly autoreactive unresponsive clones|
RNA was extracted from batch sorted CD19+CD21+CD10-CD27- and CD19+CD21-CD10-CD27- naïve B cells isolated from donors using the Absolutely RNA microprep kit (Stratagene). 100-200 ng of RNA was obtained per sample, and the quality of the purified RNA was assessed by the Bioanalyzer from Agilent. Using the Ovation biotin system kit from Nugen, 30-50ng of RNA was amplified and labeled to produce cDNA. Labeled cDNA was hybridized on chips containing the whole human genome (Human Genome U133 2.0 from Affymetrix). Data from CD21+ and CD21- B cell populations were compared in order to determine the gene signature of the newly described CD21- B cells.