
Similar protocols
Protocol publication
[…] Hippocampi were dissected and homogenized in RIPA (500 μl). Metabolites were extracted from 100 μl of hippocampal homogenate and untargeted metabolomics was completed as described. Metabolomics data was processed using MarkerView and Peakview software (AB SCIEX) and Metaboanalyst. Metabolite identification was based on mass error (<30 ppm) and MS/MS fragment ions. Many metabolites were further confirmed using retention time and comparison to authentic standards ( ± 1 min) from an in-house library (IROA Technologies). LipidMaps, METLIN and HMDB databases were used for MS and MS/MS matching. Metaboanalyst heatmap and pathway analyses were performed as described. For integrated pathway analyses, the parameters were set to ‘global test’ and ‘relative betweenness centrality’. […]
Pipeline specifications
Software tools | MarkerView, MetaboAnalyst |
---|---|
Databases | HMDB METLIN |
Application | MS-based untargeted metabolomics |
Organisms | Mus musculus |
Diseases | Alzheimer Disease, Dementia, Diabetes Mellitus, Metabolic Diseases, Neoplasms, Adipose Tissue |
Chemicals | Pentosephosphates, Purines, Glutamic Acid |