Dataset features


Application: Gene expression microarray analysis
Number of samples: 10
Release date: Aug 5 2017
Last update date: Jan 24 2018
Access: Public
Chemicals: Cholesterol, Fatty Acids
Dataset link Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes [MG_U74Cv2]

Experimental Protocol

Transgenic mice that overexpress transcriptionally active nuclear forms of human SREBP-1a (TgSREBP-1a) or SREBP-2 (TgSREBP-2) and mice that lack SCAP (Scap–/–) in liver were used. Studies included five 12- to 16-wk-old male TgSREBP-1a and TgSREBP-2 mice and five male littermate wild-type controls. Mice were fed a high protein/low carbohydrate diet for 2 weeks before liver removal to elicit high-level expression of the nSREBPs. Studies using liver-specific Scap–/– mice included five 10- to 12-wk-old male Scap–/–;MX1-Cre and corresponding wild-type mice that received four i.p. injections of polyinosinic-polycytidylic acid to induce the Cre recombinase. Mice were fed Teklad chow and livers removed 14 days after the last injection. Liver RNA was prepared using RNA STAT-60 (Tel-Test, Friendswood, TX). Equal aliquots of total RNA from each of five mice per group were pooled (total, 20 μg) and used for biotin labeling as described in the Affymetrix technical bulletin. Hybridization, washing, scanning, and analysis of the Affymetrix GeneChip Murine Genome MU74A, -B, and -C version 2 arrays (Affymetrix, Santa Clara, CA) were carried out. Duplicate hybridizations were performed with each RNA sample and data processed with MICROARRAY SUITE 5.0 (Affymetrix) software.








J Corton