Computational protocol: A novel role of the soybean clock gene LUX ARRHYTHMO in male reproductive development

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Protocol publication

[…] Protein sequences of different plant species were retrieved from NCBI (https://www.ncbi.nlm.nih.gov/) and soybean genome database at Phytozome (https://www.phytozome.net/). Sequences were aligned in the multiple sequences alignment tool CLUSTALX2.0, using Gonnet Protein Weight Matrix with default parameters. The phylogenetic tree was constructed by the Neighbour-Joining algorithm using the MEGA 5.0. The bootstrap consensus tree was inferred from 1000 replicates. […]

Pipeline specifications

Software tools Clustal W, MEGA
Databases Phytozome SoyGD
Application Phylogenetics
Organisms Glycine max, Arabidopsis thaliana