Dataset features

Specifications


Application: sRNA-seq analysis
Number of samples: 4
Release date: Sep 28 2016
Last update date: Dec 14 2016
Access: Public
Chemicals: Flavonoids, Glucose
Dataset link Integrated RNA-seq and sRNA-seq analysis reveals miRNA effects on secondary metabolism in Solanum tuberosum L [miRNA-seq]

Experimental Protocol


Four RNA samples were used in this experiment. Eight-week-old plantlets were shifted to the dark 3 day and subsequently exposed to red light (~19.7μmol m−2s−1, 630 nm). Then, the leaves of plantlets were collected and immediately frozen in liquid nitrogen after 12, 24, and 48 h, respectively. Equal amounts of total RNA were pooled at 12, 24 and 48 h time points to produce one sample. Dark-grown plantlets were collected as the control. In vitro generated microtubers were treated under the same conditions as described previously. Please note that the leaves of plantlets were collected and immediately frozen in liquid nitrogen after 12, 24, and 48 h, respectively and equal amounts of total RNA from three time points were pooled to produce one sample. The pooling strategy was employed to reduce the effect of circadian regulation on genes expression. Microtubers were treated under the same conditions and strategy.

Repositories


GEO

GSE79984

ENA

SRP072936

BioProject

PRJNA317570

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