Computational protocol: Cold-pressed minke whale oil reduces circulating LDL/VLDL-cholesterol, lipid oxidation and atherogenesis in apolipoprotein E-deficient mice fed a Western-type diet for 13 weeks

Similar protocols

Protocol publication

[…] Immediately after blood drainage, all mice were perfused through the left ventricle with sterile saline (0.9%), until no residual blood was apparent in the perfusate (approximate 5 min perfusion). The entire aorta (proximal ascending the aorta to bifurcation of the iliac arteries) was cleaned in situ of periadventitial fat, dissected and fixed in 1% paraformaldehyde solution. Finally, the aorta was stained by Oil Red O staining, opened longitudinally and en face-mounted on slides as previously described []. After 48 h rest, the slides were scanned with a high-resolution scanner. The lesion areas were evaluated using ImageJ software [] and the extent of atherosclerosis was reported as the percentage of the total area of a given aortaor an aortic region occupied by atherosclerotic lesions. [...] All of the statistical analyses were performed using IBM SPSS Statistics for Macintosh (Release, SPSS, Inc., Chicago, IL, US). The Shapiro-Wilk’s test was performed to determine the distribution of the variables and non-normally distributed variables were log-transformed before statistical analysis. Statistical difference was assessed using One-Way ANOVA with Tukeys’ post hoc test or Kruskal-Wallis test. The relative gene expression was analysed with REST 2009 (V2.0.13) []. A value of p < 0.05 was considered statistically significant. […]

Pipeline specifications

Software tools ImageJ, SPSS
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Balaenoptera acutorostrata, Mus musculus, Homo sapiens, Zea mays subsp. mays
Diseases Cardiovascular Diseases, Encephalomyelitis, Western Equine, Atherosclerosis
Chemicals Cholesterol, Fatty Acids