Dataset features

Specifications


Application: RNA-seq analysis, sRNA-seq analysis
Number of samples: 3
Release date: Apr 30 2011
Last update date: Jul 24 2014
Access: Public
Taxon: Zea mays
Dataset link High-throughput sequencing of small RNAs in Chara corallina

Experimental Protocol


Small RNA libraries were derived from thallus of Chara corallina incubated in different growth conditions. Total RNA was isolated using the Plant RNA Purification Reagent (Invitrogen) and submitted to Illumina (Hayward, CA, http://www.illumina.com) for small RNA library construction using approaches described in (Lu et al., 2007) with minor modifications. The small RNA libraries were sequenced with the Sequencing-By-Synthesis (SBS) technology by Illumina. PERL scripts were designed to remove the adapter sequences and determine the abundance of each distinct small RNA. We thank John Boyer for providing the plant material, as well as Kan Nobuta and Gayathri Mahalingam for assistance with the computational methods.

Repositories


GEO

GSE28752

ArrayExpress

E-GEOD-28752

BioProject

PRJNA143105

Download


Contact


Emanuele De Paoli