Computational protocol: Within- and Between-Species Variation of Wing Venation in Genus Monochamus (Coleoptera: Cerambycidae)

Similar protocols

Protocol publication

[…] Coordinates of the landmarks were analyzed using tpsRelw software (). There was a single orthogonal generalized Procrustes superimposition of all wings to the average configuration. The natural logarithm of the centroid size was used as a measure of wing size and it was analyzed using factorial analysis of variance (ANOVA) in Statistica (). Unless specified otherwise, this software package was used in all other statistical analysis. Wing shape was described using partial warp scores, and it was analyzed using multiple analysis of covariance (MANCOVA) with wing size as a covariate. This allowed allometry to be taken into account. To describe the differences in wing shape between groups, squared Mahalanobis distance (MD) was used. Discrimination between species was based on the canonical variates analysis (CVA) of wings shape. In all cases, leave-one-out cross-validation in PAST software () was used to calculate classification rates. The similarity between species was compared using the UPGMA method in PHYLIP software (). The tree was based on squared MDs between species including both males and females. The Bialowiezan population of M. sartor and M. urussovii was excluded from the UPGMA. […]

Pipeline specifications