Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Sep 30 2016
Last update date: Jul 26 2018
Access: Public
Diseases: Celiac Disease
Chemicals: Biotin
Dataset link Celiac disease T cell clone response to CD3/CD28 stimulation

Experimental Protocol


CD4+ T clone L10 cells, isolated from a duodenal biopsy of a celiac disease patient (Petersen et al., 2014, Nat. Struct. Biol. 21:480-488) were stimulated for 3 hours in IMDM with plate-bound CD3/CD28-specific (2.5 µg/ml each) or control antibodies. Three independent biological replicates were performed, each time including 6 million Ficoll-purified live cells per condition.

Repositories


GEO

GSE73599

BioProject

PRJNA297406

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Contact


Martijn Brugman