Computational protocol: Modulation of mTOR Signalling Triggers the Formation of Stem Cell-like Memory T Cells

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Protocol publication

[…] The raw sequencing reads were trimmed with Trim Galore! (http://www.bio-informatics.babra-ham.ac.uk/projects/trim_galore/) (Version 0.3.3), using cutadapt (Version 1.2.1) to remove low-quality bases (quality Phred score cut-off 15) and remaining adaptor sequences. Further information is provided in the Supplemental Experimental Procedures. For all analyses, a p-value (adj.) less than 0.05 was considered as statistically significant and labelled with *, less than 0.01 with **, less than 0.001 with *** and less than 0.0001 with ****. […]

Pipeline specifications

Software tools Trim Galore!, cutadapt
Application RNA-seq analysis
Organisms Homo sapiens
Diseases Neoplasms
Chemicals Sirolimus