Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Jun 13 2008
Last update date: Aug 28 2018
Access: Public
Diseases: Anemia, Anemia, Hypochromic
Chemicals: Iron
Dataset link Microarray data of mouse fetal liver

Experimental Protocol


Isolated Ter119-positive fetal liver cells from three to five E14.5 embryos of the same genotype were combined, and total RNA was extracted using TRIzol reagent (Life Technologies) with two additional ethanol precipitations. RNA quality was verified using an Agilent Bioanalyzer (Agilent Technologies). Microarray analyses were performed using Affymetrix Mouse Genome 430 2.0 array GeneChips (Affymetrix). Up- and down-regulated genes were selected based on P values of 1.5 or -1.5 as assessed by ANOVA using Partek Pro software (Partek). To determine specific pathways, gene pathway analysis was obtained using the statistically significant gene list (P<0.05) represented on the chip.

Repositories


GEO

GSE11777

ArrayExpress

E-GEOD-11777

BioProject

PRJNA105965

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Contact


Bing Mei Zhu