|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Jun 13 2008|
|Last update date:||Aug 28 2018|
|Diseases:||Anemia, Anemia, Hypochromic|
|Dataset link||Microarray data of mouse fetal liver|
Isolated Ter119-positive fetal liver cells from three to five E14.5 embryos of the same genotype were combined, and total RNA was extracted using TRIzol reagent (Life Technologies) with two additional ethanol precipitations. RNA quality was verified using an Agilent Bioanalyzer (Agilent Technologies). Microarray analyses were performed using Affymetrix Mouse Genome 430 2.0 array GeneChips (Affymetrix). Up- and down-regulated genes were selected based on P values of 1.5 or -1.5 as assessed by ANOVA using Partek Pro software (Partek). To determine specific pathways, gene pathway analysis was obtained using the statistically significant gene list (P<0.05) represented on the chip.
Bing Mei Zhu