Computational protocol: Drug-sensitive FGFR3 mutations in lung adenocarcinoma

Similar protocols

Protocol publication

[…] FASTQ files were analyzed using BWA-__STRONG_START__Picard-__STRONG_START__GATK/MuTect pipeline generating 3349 unique variants (, available at Annals of Oncology online). Polymorphisms overlapping with dbSNP database (v.142) and Indian specific SNP database TMC-SNPdb derived from whole exome sequencing of 62 normal samples [] were filtered (, available at Annals of Oncology online). Stringent mutation analysis was carried out as further detailed in , available at Annals of Oncology online to derive list of significant mutations for further validation (, available at Annals of Oncology online). […]

Pipeline specifications

Software tools BWA, Picard, GATK, MuTect
Databases dbSNP TMC-SNPdb
Application WES analysis
Organisms Homo sapiens/Mus musculus xenograft, Mus musculus, Homo sapiens
Diseases Lung Neoplasms, Neoplasms, Myoclonic Epilepsies, Progressive, X-Linked Combined Immunodeficiency Diseases