|Application:||Gene expression microarray analysis|
|Number of samples:||12|
|Release date:||Oct 9 2008|
|Last update date:||Feb 20 2014|
|Dataset link||Global profiling of B. subtilis comparing wild-type to mutants with altered cytoplasmic membrane composition|
WT vs.ugtP single mutant (U), WT vs. mprFpssAywnE triple mutant (T) or WT vs. ugtPmprFpssAywnE quadruple mutant (Q). Each experiment (WT vs. mutant) was conducted four times using three independent total RNA preparations (3 independent experiement + dye swap). For the 3 datasets used for final analysis wild-type was labeled with Alexa Fluor 555 and mutants with Alexa Fluor 647. For dye swap experiments wild-type was labeled with Alexa Fluor 647 and mutants with Alexa Fluor 555.
John D. Helmann