Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Oct 9 2008
Last update date: Feb 20 2014
Access: Public
Chemicals: Cardiolipins, Phosphatidylglycerols
Dataset link Global profiling of B. subtilis comparing wild-type to mutants with altered cytoplasmic membrane composition

Experimental Protocol


WT vs.ugtP single mutant (U), WT vs. mprFpssAywnE triple mutant (T) or WT vs. ugtPmprFpssAywnE quadruple mutant (Q). Each experiment (WT vs. mutant) was conducted four times using three independent total RNA preparations (3 independent experiement + dye swap). For the 3 datasets used for final analysis wild-type was labeled with Alexa Fluor 555 and mutants with Alexa Fluor 647. For dye swap experiments wild-type was labeled with Alexa Fluor 647 and mutants with Alexa Fluor 555.

Repositories


GEO

GSE13036

ArrayExpress

E-GEOD-13036

BioProject

PRJNA109709

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Contact


John Helmann
John D. Helmann