Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 207
Release date: Dec 30 2014
Last update date: Apr 1 2015
Access: Public
Diseases: Malaria
Dataset link Effect of Antigen, Type I IFN and STING Signaling on CD8 T Cell Immunity With Adenoviral Vaccines

Experimental Protocol


Each microarray comes from an RNA sample obtained from a unique mouse. There were no repeated RNA samples obtained from any mouse. 207 arrays were run overall, 189 from dLN and 18 from PBMCs obtained from mice vaccinated with different rAd vectors. The dLN arrays were run in three batches, denoted A-C, and the PBMCs were run in Batch D. Batch A (108 arrays)consisted of six biological replicates at each of three time points (8, 24 and 72 hours) post-vaccination with PBS, rAd5 (1 x 10^8 PU), rAd28 (1 x 10^8 PU), rAd35 (1 x 10^9 PU), sAd11 (1 x 10^8 PU) and sAd16 (1 x 10^8 PU). Batch B (46 arrays) consisted of four biological replicates at each of three time points (8, 24 and 72 hours) post-vaccination with chAd3 (1 x 10^8 PU), chAd63 (1 x 10^8 PU) and Poly I:C. Batch B also consisted of two biological replicates at each of three time points (8, 24 and 72 hours) post-vaccination with rAd5 (1 x 10^8 PU) and four biological replicates at each 8 hour time point post-vaccination with PBS (control). Batch C (35 arrays) consisted of five biological replicates at each 8 hour time point post-vaccination of WT, IFNabR-/- and STING gt/gt mice with PBS and chAd63 (3 x 10^7 PU). Batch C also consisted of five biological replicates at each 8 hour time point post-vaccination with rAd5 (3 x 10^7 PU). Batch D (18 arrays) consisted of the PBMC microarray set, which included six biological replicates at each 24 hour time point post-vaccination with PBS, rAd5 and rAd28.

Repositories


GEO

GSE64548

BioProject

PRJNA271285

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