
Similar protocols
Protocol publication
[…] Genomic sequence of miRNA cluster and the predicted transcription factor binding sites were extracted from the "Cis-element clusters within BlastZ Aligments" option at the GenomeTraFac []. Tissue distribution of selected genes was derived from the GNF database [], in which the expression data from the same tissue types examined in our study were extracted for analyses. Target genes of miRNAs were predicted using the miRanda open-source software [] associated with the miRBase [] with a cutoff p value less than 0.05. The IGTC database [] was used to search the phenotype of mice carrying loss-of-function mutations. […]
Pipeline specifications
Software tools | LASTZ, MiRanda |
---|---|
Databases | miRBase GenomeTrafac IGTC |
Application | Nucleotide sequence alignment |
Organisms | Homo sapiens |