Dataset features

Specifications


Application: Gene expression microarray analysis, miRNA array analysis
Number of samples: 16
Release date: Jan 10 2015
Last update date: Oct 20 2015
Access: Public
Diseases: Lymphoma, Follicular
Dataset link microRNA expression profiling of different T cell subset in human tonsils

Experimental Protocol


RNA was extracted from 4 separate human tonsils (biological replicates) using mirVANA RNA isolation kit (Ambion) according to the manufacturer’s protocol. Each biological sample was analysed individually. Live lymphocytes were sorted based on negative 7AAD staining, and thefollowing four subsets were purified: Naive T cells (CD4+ CD45RO-); CD57+ Tfh cells (CD4+ CD45RO+ PD-1_high CXCR5_high CD57+); CD57- Tfh cells (CD4+ CD45RO+ PD-1_high CXCR5_high CD57-) and non-Tfh effector or memory T cells (CD4+ CD45RO+ PD-1_neg CXCR5_neg). RNA quantity and quality was determined using a Nanodrop 1000 and an Agilent 2100 Bioanalyzer respectively. Hybridization on Agilent Human miRNA Microarray (V1) Kit, 8x15K and was performed at The Ramaciotti Center for Genomics (University of New South Wales, Australia). The mean of 3-4 biological replicates from each population of each population were calculated and values were plotted using Prism.

Repositories


GEO

GSE64833

ArrayExpress

E-GEOD-64833

BioProject

PRJNA272140

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Contact


Monika Srivastava

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