|Application:||Gene expression microarray analysis|
|Number of samples:||51|
|Release date:||Jan 30 2013|
|Last update date:||Nov 6 2013|
|Diseases:||Heart Diseases, Heart Failure|
|Dataset link||Expression data from cyclically stretched engineered neonatal rat ventricuar myocyte (NRVM) tissues|
NRVMs were seeded on silicone membranes coated with isotropic (iso) fibronectin (FN) or micropatterned with FN (aniso), cultured statically for 1h (t=0h), and stretched for increasing amount of time (t=6h, 24h, 96h) before RNA extraction and hybridization on Affymetrix microarrays. For aniso tissues, stretch was applied in either the longitudinal (long) or transverse (trans) direction. RNA was collected over six primary NRVM harvests and thus RNA was also extracted and analyzed from samples seeded for 1h (at t=0h) on iso FN to be used to normalize across cell harvests.
Megan Laura McCain