Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 51
Release date: Jan 30 2013
Last update date: Nov 6 2013
Access: Public
Diseases: Heart Diseases, Heart Failure
Dataset link Expression data from cyclically stretched engineered neonatal rat ventricuar myocyte (NRVM) tissues

Experimental Protocol


NRVMs were seeded on silicone membranes coated with isotropic (iso) fibronectin (FN) or micropatterned with FN (aniso), cultured statically for 1h (t=0h), and stretched for increasing amount of time (t=6h, 24h, 96h) before RNA extraction and hybridization on Affymetrix microarrays. For aniso tissues, stretch was applied in either the longitudinal (long) or transverse (trans) direction. RNA was collected over six primary NRVM harvests and thus RNA was also extracted and analyzed from samples seeded for 1h (at t=0h) on iso FN to be used to normalize across cell harvests.

Repositories


GEO

GSE43846

ArrayExpress

E-GEOD-43846

BioProject

PRJNA187574

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Contact


Megan McCain
Megan Laura McCain