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[…] 13C, 15N and GSSG 13C,15N. NAA‐d3 was purchased from CDN isotopes, all other itotopically labeled compounds from Toronto Research Chemical, Canada. LC‐MS grade solvents were used for sample preparation. Overall, the TEC values were comparable for the different regions (mutant or wild‐type tumor, contralateral brain, and control brain), indicating that for the majority of compounds, the quantification is not likely to be affected by strong tissue effects (). Except for GSSG, which was excluded from the present data, since all isotopic compounds were simultaneously present in the mix, oxidation reactions cannot be excluded, which may explain differences in GSSG values. Data were treated with Fleximaging 4.0 (Bruker, Germany), proprietary softwares Quantinetix™ 1.7, and Multimaging™ 1.0.30 (ImaBiotech, Loos, France)., LC‐MS analysis was run on snap‐frozen tissue of six PDX samples and 13 clinical samples. Each sample was analyzed in three replicates. For metabolite extraction, a 5‐mm metal bead (Qiagen, No69989) and extraction buffer (methanol/acetonitrile/water‐50/30/20) containing 100 ng/ml HEPES for internal standard purpose (Sigma, H4034) were added to the tissue at a volume/weight ratio of 25 μl/mg. The tissue was disrupted in a bead mill (Qiagen, Tissuelyzer, Hombrechtikan, Switzerland) with 2 cycles (2 × 20 s/20 MHz) before vortexing on an Eppendorf Thermomixer at 2°C/20 min/1,400 rpm. The extract was clarified by centrifugation (15 min/13,362 g/4°C […]

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