Computational protocol: Detection of relapsing fever Borrelia spp., Bartonella spp. and Anaplasmataceae bacteria in argasid ticks in Algeria

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Protocol publication

[…] Phylogenetic trees were drawn using the neighbor-joining method from an alignment of the different genes used in the experiments. Sequences were aligned using CLUSTALW, and phylogenetic inferences obtained using the ML phylogenetic analysis with the TOPALi 2.5 software (Biomathematics and Statistics Scotland, Edinburgh, UK) within the integrated ML application, using the K81uf + I + Г substitution model. Numbers at the nodes are percentages of bootstrap values obtained by repeating the analysis from 100 replicates to generate a majority consensus tree. […]

Pipeline specifications

Software tools Clustal W, TOPALi
Application Phylogenetics
Organisms Homo sapiens, Ornithodoros capensis
Diseases Borrelia Infections, Relapsing Fever