Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Apr 2 2011
Last update date: Mar 20 2017
Access: Public
Taxon: Homo sapiens, Mus musculus
Dataset link Generation of Induced Pluripotent Stem Cells from Human Testis Biopsy Derived Fibroblastic Cells

Experimental Protocol


For transcriptome profiling, 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h). Quality-checked cRNA samples were hybridized as biological or technical duplicates for 18 h onto human-8 v3 expression BeadChips (Illumina), washed, stained, and scanned following guidelines and using materials/instrumentation supplied/suggested by the manufacturer. 8 samples were analyzed: NCL: NCL3 hESCs grown on feeders, bulk harvest at semi-sub confluence (1 replicate) HuES6: HuES6 hESCs grown on feeders, bulk harvest at semi-sub confluence (1 replicate) HT3: Human fibroblast-like testis cells, line 3 (1 replicate) HT16: Human fibroblast-like testis cells, line 16 (1 replicate) FS: FS human foreskin fibroblasts (1 replicate) BJ: BJ human fibroblasts (1 replicate) iPS3: hiPSC line 2 derived from HT3 (1 replicate) iPS16: hiPSC line 2 derived from HT16 (1 replicate)

Repositories


GEO

GSE21179

ArrayExpress

E-GEOD-21179

BioProject

PRJNA126553

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Contact


Marcos Ara├║zo-Bravo
Marcos J Arauzo-Bravo