Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Dec 1 2006
Last update date: Oct 29 2018
Access: Public
Diseases: Lung Neoplasms, Neoplasms
Dataset link Cultured A549 lung cancer cells treated with actinomycin D and sapphyrin PCI-2050

Experimental Protocol


A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eight days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, sapphyrin PCI-2050 (1.25 or 2.5 μM final concentration), actinomycin D (5 μg/mL final concentration), or control (5% mannitol) solution was added to the cultures. Each experiment was performed in triplicate. After incubation, all cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated and subjected to analysis on Human Genome U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA).

Repositories


GEO

GSE6400

ArrayExpress

E-GEOD-6400

BioProject

PRJNA99711

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Contact


Joseph Hacia
Joseph Gerard Hacia

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